Respondent
Theme
Changes in biophysical and morphological parameters of loach embryos for actions of flurenizyd
Defence Date
Annotation
Dissertation is dedicated to the research of the free-radical reactions (in vitro and in
vivo) of loach embryos for actions of flurenizyd – preparation of antimicrobial,
antituberculousis, antichlamydia, immunomodulator, antioxidant, hepatoprotective,
antiinflammatory, antiviral action. Influence of flurenizyd is studied on functioning of
Na+, K+-АТPаse of loach embryos Misgurnus fossilis L. on the stages of development 2,
16, 64, 256 and 1024 blastomeres. Flurenizide in all studied concentrations (0.01; 0.05;
0.15; 1; 5; 15 mM) caused increase in content of lipid peroxidation primary products
(hydroperoxides) during development of embryos but decrease on stage 256 blastomeres.
The article contains data on ultrastructural and morphological changes of embryos
of loach Misgurnus fossilis L., what developed in incubation environment, which contain
flurenizyd. It is shown that an antibiotic is in the concentrations of 1; 5; 15 mМ
predetermines the high death rate of embryos of loach already on the first time of his
action. It is set that flurenizyd causes structural violations of mitohondria and cytoplasmic
net, causes the increase of amount of lysosomes at the embryos of loach on the stage of
development of 2 blastomeres. A degree of expressed of these changes is dosedependent.
The obtained results extend understanding of the mechanisms of flurenisid action on
membrane processes of embryonic cells. These data can be used for toxicological
evaluation of bioactive or pharmacological substances, and therefore have value for human
and veterinary medicine.
After a two-factor analysis of variance for the first time found that the formation of
primary products of lipid peroxidation (hydroperoxides) has a significant effect of loach
embryos. However, the formation of secondary products of lipid peroxidation
approximately the same effect commit time and flurenizyd and unmeasured factors.
At superoxidedismutase, catalase, glutathioneperoxidase and glutathione-S-
transferase activity of loach embryos exert a powerful influence unmeasured factors,
which may include external factors in which development occurs. We found that the
development is more pronounced effect on catalase activity.
Applying cluster analysis was first found to be under 64 blastomeres for all
experimental groups studied parameters were divided into 4 groups of similarity. Revealed
that the concentration of flurenizyd 0,05mM not have a significant impact on performance,
the value of which was at the level of control (at 2 blastomeres). At the stage of 256 and
1024 all blastomeres study group on indicators are divided into 5 groups of similarity that
indicates a change flurenizyd impact on those stages of loach embryos.
The research results are important for flurenizyd use in the treatment of pregnant
women as well as for further research on its effects on reproductive function. The
conclusions arising from the research results can be used in the formation of new
regulations related to pharmacology.
Keywords: loach embryos, flurenizyd, embryogenesis, blastomeres, lipid
peroxidation, antioxidant enzymes system, plasma membrane, Na+, K+-ATPase.