Information on the thesis

Thesis for PhD degree, specialty 03.00.04 – biochemistry. – Ivan Franko National
University of Lviv, Lviv, 2015.
This dissertation was devoted to the estimation of rheumatoid arthritis pathogenesis
mechanisms  in  experimental  animals.  Two  models  of  experimental  inflammation  were
used in the study: acute, caused by carrageenаn and chronic, collagen-induced arthritis. It
has been revealed that during the acute inflammation with hyperproduction of α1 and α2-
globulin,  inflammatory  processes  were  more  pronounced  than  during  chronic  collagen-
induced  arthritis.  The  increase  of  gammaglobulin  fraction  during  chronic  inflammatory
process  indicates  the  involvement  in  the  development  of  pathological  process  of  the
humoral link of immunity with hyperproduction of autoantibodies. In carrageenan-induced
immunoinflammatory arthritis was a significant activation of phagocytic cells, with some
increasing after stimulation was observed. In the case of experimental collagenassociated-
arthritis  phagocytes  were  able  to  increase  their  functional  ability  after  stimulation.
Сollagen  arthritis  was  revealed  a  significant  intensification  of  LPO  and  improved  the
content  of  stable  metabolites  of  nitric  oxide,  indicating  the  substantial  generation  of
reactive oxygen and nitrogen. Some predominance of NO-synthase pathway compared to
arginase  pathway  were  detected  (oxidative  way  over  nonoxidative).  The  acute
inflammation was characterized by a decrease in a number of apoptotic lymphocytes in the
blood of experimental rats and chronic inflammation was characterized with their increase
compared  with  control  (non-immunized)  rats.  It  had  been  estimated  that  antіhiston  H1
IgG-antibodies have some proteolytic activity against histone H1 and myelin basic protein.
It had been established that the proteolytic  activity of IgG on histone was detected in the
serum of patients with rheumatoid arthritis, but there was no IgG in the serum of healthy
donors. This makes the IgG fraction as a potential biochemical marker for predicting of
rheumatoid arthritis development.
Keywords:  rheumatoid  arthritis,  carrageenan,  collagen-induced  arthritis,  reactive
oxygen and nitrogen, apoptosis, proteolytic activity