Information on the thesis

Dissertation  for  the  degree  of  candidate  of  biological  sciences,  field  03.00.04
biochemistry. – Ivan Franko National University of Lviv, Ministry of Education and Science of
Ukraine, Lviv, 2014.
The  main  purpose  of  dissertational  work  was  to  research  biochemical  effects  of
alkaloid-free  Galega  officinalis  L.  fraction  on  structural  and  functional  state  of  rats’
leucocytes  and  pancreatic  cells  under  conditions  of  diabetes  mellitus  induced  by
streptozotocin.
A method of alkaloid-free fraction from Galega officinalis stabilization by addition of
biological  surface  active  agent  synthesized  by  Pseudomonas  sp.  PS-17  bacteria  was
developed. Using gas chromatographic-mass spectrometric method were found component
composition  of  stabilized  alkaloid-free fraction from  Galega officinalis, which includes 31
compound, 26 of them were identified. Component composition include higher fatty acids
and their esters (63.90% of all components), monoterpenes – loliolid (1.43%), diterpenes –
Neophytadiene  (1,  93%)  and  phytol  (3.675%),  triterpenes  –  squalene  (0.343%)  and  α-
amyrine  (1,932%),  phytosterols  –  campesterol  (1.966%)  and  stigmasterol  (14.725%),
flavonoids (2.98%), vitamin E (0,46%).
Pronounced  hypoglycemic  effects  of  stabilized  alkaloid-free  fraction  from  Galega
officinalis (at a dose of 0.6 g/kg) and its cell protective effects on Langerhans islet cells of
the  pancreas  system  (at  a  dose  of  1.2  g/kg)  over  a  model  of  streptozotocin-induced
diabetes  were  proved.  A  statistically  significant  increase  in  the  density  index  of  the
Langerhans  islets,  their  individual  parameters  and  the  number  of  incretory  cells  are  as
evidence of these effects. Relative maintaining β-cells, with features of minor cytoplasm
vacuolization and decreased levels of degranulation were detected. Stabilized alkaloid-free
fraction from Galega officinalis  protective effect  on  pancreas’  β-cells  in  case of  diabetes
mellitus is confirmed by the increase of C-peptide and insulin concentrations in the blood
plasma of rats that was found in the study. Also it was revealed, that Stabilized alkaloid-
free  fraction  from  Galega  officinalis  prevents  the  development  of  oxidative  stress  under
experimental  diabetes  conditions  due  to  the  ability  of  its  components  scavenging  free
radicals and to prevent inhibition of main  antioxidant enzymes. Depressive influence of
Stabilized  alkaloid-free  fraction  from  Galega  officinalis  on  leucocytes  apoptosis,  the
intensity of which increases notably under conditions of diabetes mellitus was shown. A
corrective influence on the proliferation and differentiation of leucocytes’ precursor cells,
on actin polymerization process of these cells and on TNF-α content was also discovered
in our studies of pathology.
Key words: diabetes mellitus, alkaloid-free Galega officinalis L. extract, oxidative
stress, apoptosis, actin polymerization, leucocytes, pancreatic β-cells.